AKTA FPLC-own column and buffers

$50

$80

AKTA FPLC

$90

$120

Tycho (NanoTemper Thermofluorescence)

$1

$11

HPLC_MALS per run charge only + staff time

staff only

$120

ITC

$30

$40

Biacore X100 per day -no analysis

Staff Only

$380

Biacore X100 per day-user supplies matrix chip

Staff Only

$140

Scorpion-user provides reagents

$30

$50

Gryphon Robot Setup- 96 well crystal screen

$80

$90

Grypohon Robot Setup- 96 well additive screen

$90

$100

Sonicator*

$10

$20

Avestin*

$60

$90

Sorvall medium Speed*

$40

$60

Beckman Ultra *

$50

$60

Shaker *

$10

$40

SDS PAGE (1 gel, Coomassie stain, digital scan)*

$20

$50

Western (user-supplied or in-stock Ab)*

$30

$70

Consumable Unit Cost*

$10

 In-House micro-max 007 setup

$30

 $30

In-House micro-max 007 beamtime per hour *

 $40

 $60

Synchrotron data collection at Synchrotron per sample

 $10

 $20

 SAXS data collection at Synchrotron per sample

 $15

 $15

Data analysis, structure determination, training, consultation; hourly rate*

 $45

Protein Expression 2L LB, SDS-PAGE, and Western Confirmation

$410

Protein Expression 6L LB, SDS-PAGE, and Western Confirmation

$550

Protein Expression 5L bioreactor LB, SDS-PAGE, and Western Confirmation.

$520

Protein purification 2L, 1 step purification

$570

Protein purification 6L, 1 step purification

$940

Additional purification step, SDS-PAGE

$170

Insect Cell, Cellfectin Transfection 6 well plates, to 50 mL P1, SDS-PAGE, Western

$358

Insect Cell, virus P2-P3 250 mL, SDS-PAGE, Western

$364

Insect Cell Virus 1L Infection, Express 5, freeze in -80C

$329

Insect Cell Protein 1L prep (one step purification)

$565

HEK Cell 30 mL test Expression, western to confirm expression

$570

HEK Cell 200 mL Expression (secreted)

$600

HEK Cell 200 mL Purification (secreted), 2 step purification

$570