Equipment

Macromolecular Crystallography [ISB 016A]

Rigaku MicroMax 007HF X-ray generator with VariMax HighFlux optic. Pilates Dectris 200K detector and AFC Kappa 4-circle goniometer
Oxford liquid nitrogen crystal cryo-cooling system with auto refill
Art Robbins Instruments GRYPHON liquid-handling crystallization robot
Scorpion Matrix maker for custom crystallization grid preparation
Crystallization kits, including Hampton, Qiagen, JCSG, and Wizard Suites
JANSi visible/UV fluorescence digital microscope for crystal viewing and documentation
Olympus microscope with variable objectives for crystal viewing and documentation
Zeiss Stemi SU8 stereomicroscope for crystal harvesting
3 low-vibration incubators (4-20°C) for storage of crystallization experiments
GEForce RTX 2080 4-node GPU workstation for cryoEM and crystallographic analysis
Linux workstation, installed with crystallographic software suites, is available for in-house and remote diffraction data processing and structure determination
HKL2000 and CrysAlis^PRO software for data processing and scaling
CCP4, PHENIX, COOT, and PYMOL software packages for crystal structure determination and visualization
ATSAS software package for small-angle X-ray scattering data analysis
RELION, CryoSPARC, EMAN2 and IMOD software for Cryo-EM single particle structure determination and tomography

Thermo Scientific Barnsted NanoPure water purification system
Beckman Coulter Optima XE-90 ultracentrifuge with 2 Beckman Coulter Ti45 rotors
Sorvall RC6+ Floor centrifuge with Fiberlite F10 4x10000 LEX and Fiberlite F21-8x50Y rotors
Sorvall RT7 bench-top centrifuge
BenchMark bench-top incubator/shaker
LabNet PrismR benchtop refrigerated microcentrifuge
Thermo Scientific Legend Macro 21 PCR cycler
3-door 4^oC Deli fridge
Three 4°C laboratory refrigerators (one dedicated to ISB016C)
-20°C laboratory freezer
VWR -80°C laboratory freezer

Two stacked Innova 4430 refrigerated floor shakers for bacterial growth
Barnstead/Labline MaxQ/400 and benchtop orbital shaker
Brunswick Bioflo/Celligen 115 fermenter with Eppendorf 7L vessel and Thermoflex 1400 recirculating water bath
Thermo Fisher Isotemp incubator for bacterial growth

SterilGard III Advance Class II Biosafety Cabinet
Two stackable Innova 4430 temperature-controlled incubator shakers for maintenance and scale-up insect cell growth
Thermo Forma Direct Heart CO₂ Incubator with HEPA filter for batch mammalian cell culture, equipped with orbital shakers
Thermo Forma MIDI 40 temperature controlled CO₂ incubator for mammalian cell passaging
Nikon Eclipse Ti-SM inverted microscope
Left Technology Countess II cell counter
Binder Drying Oven for baking sterilized culture flask

2 GE AKTA-Pure fast performance Chromatography System for high-throughput protein purification, with 1 Shimadzu RF-20Axs Prominence fluorescence detector
Avestin Emulsiflex C5 High Pressure Homogenizer for cell lysis
Large collection of commercial pre-packed and in-house packed affinity, ion-exchange, hydrophobic interaction and size-exclusion chromatographic columns

Microcal VP-ITC Calorimeter for determination of binding constants of protein-protein or protein-ligand interactions
Two BioRad Mini Protean slab gel electrophoresis units with power source
Thermo Scientific NanoDrop One microspectrophotometer
Two BioRad Transblot transfer cells for Western blots
Wyatt TREOS-II multi-angle light scattering detector and Optilab T-rEX Index of Refraction monitor connected in-line to output of Superdex 200 column mounted on an Agilent 1100 series HPLC system
Biacore X100 surface plasmon resonance detector for protein-ligand binding kinetics and affinity
Nano-temper Tycho NT.6 thermo-fluorescence detector for determination of protein stability
Perkin Elmer LS55 luminescence spectrometer